Thermo Fisher Scientific Human CCL7 (MCP-3) Recombinant Protein
인간 CCL7(MCP-3) 재조합 단백질로, 염증 및 전이 과정에서 대식세포 유인 연구에 사용됩니다. E. coli 발현, 98% 이상 순도, ED50 10–100 ng/mL 활성. 동결건조 형태로 제공되며, 재구성 후 -20°C 이하 보관 권장.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Human CCL7 recombinant protein contains 76 amino acids |
| Molecular Weight | 9 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >98% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 = 10–100 ng/mL; determined by the dose-dependent chemotaxis of human PBMCs |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Carrier-Free
- Reconstitution: Briefly centrifuge vial before opening. Reconstitute lyophilized protein in sterile distilled water to 0.1–1.0 mg/mL. Further dilute in a buffered solution containing carrier protein (e.g., PBS with 0.1–1.0% BSA).
- Storage: Lyophilized preparation stable at -20°C for up to 2 years. After reconstitution, aliquot and store at ≤-20°C for up to 3 months with carrier protein. Minimize freeze-thaw cycles.
Target Information
This is a secreted chemokine that attracts macrophages during inflammation and metastasis. It belongs to the C-C chemokine subfamily, characterized by two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, which degrades components of the extracellular matrix.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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