Thermo Fisher Scientific Human CCL7 (MCP-3) Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

Human CCL7 recombinant protein contains 76 amino acids

Molecular weight

9 kDa

Class

Recombinant

Type

Protein

Purity

>98% by SDS-PAGE

Endotoxin concentration

<0.1 ng/µg

Activity

ED50 = 10 - 100 ng/mL; determined by the dose-dependent chemotaxis of human PBMCs.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Wet or Dry ice (international)

Product Specific Information

Carrier-Free

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This lyophilized protein should be reconstituted in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in a buffered solution containing carrier protein, such as phosphate buffered saline supplemented with 0.1-1.0% BSA.

Storage: This lyophilized preparation is stable at -20°C for up to 2 years from date of receipt. The reconstituted MCP-3 should be apportioned into working aliquots and stored at ≤-20°C for up to 3 months with a carrier protein. Keep freeze-thaw cycles to a minimum.

Target Information

This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.