Thermo Fisher Scientific NAG-1 Polyclonal Antibody, HRP

Thermo Fisher Scientific NAG-1 Polyclonal Antibody, HRP

Applications and Tested Dilution

• Western Blot (WB): 1:1,000–1:5,000
• ELISA: Assay-dependent
• Immunoprecipitation (IP): Assay-dependent

Product Specifications

Product Specific Information

Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

This antibody reacts with endogenous NAG-1 protein from human tissues. BLAST analysis using the immunizing recombinant protein suggests reactivity with NAG-1 from human and chimpanzee (100% and 98% homology, respectively), macaque (94%), and mouse and rat (70%). Cross-reactivity with NAG-1 from other sources has not been determined.

Target Information

GDF-15 belongs to the TGF-beta cytokine family, which plays an important role in prenatal development, postnatal growth, and tissue maintenance. GDF-15 is mainly expressed in the placenta and to a lesser extent in various other tissues. Its presence in amniotic fluid and elevated levels in pregnant women suggest involvement in gestation and embryonic development. GDF-15 generally exhibits tumor suppressive activities and is secreted in response to activation of the p53 pathway. Serum levels of GDF-15 correlate with progression of several tumor types, including colorectal, pancreatic, and prostate cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.