Thermo Fisher Scientific NAG-1 Polyclonal Antibody, HRP
HRP 결합된 NAG-1 폴리클로날 항체로 Western blot, ELISA, IP 등 다양한 응용에 적합. Human 및 Mouse 반응성. Protein A로 정제된 Rabbit IgG 포맷. 장기 보관 시 -20°C에서 안정적으로 유지.
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Thermo Fisher Scientific NAG-1 Polyclonal Antibody, HRP
Applications and Tested Dilution
- Western Blot (WB): 1:1,000–1:5,000
- ELISA: Assay-dependent
- Immunoprecipitation (IP): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Protein-A purified antibody prepared by repeated immunizations with MBP-tagged recombinant protein produced in E. coli, corresponding to mature human NAG-1 protein. Cross-reactivity to MBP removed via cross-adsorption chromatography. |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C for short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody reacts with endogenous NAG-1 protein from human tissues. BLAST analysis using the immunizing recombinant protein suggests reactivity with NAG-1 from human and chimpanzee (100% and 98% homology, respectively), macaque (94%), and mouse and rat (70%). Cross-reactivity with NAG-1 from other sources has not been determined.
Target Information
GDF-15 belongs to the TGF-beta cytokine family, which plays an important role in prenatal development, postnatal growth, and tissue maintenance. GDF-15 is mainly expressed in the placenta and to a lesser extent in various other tissues. Its presence in amniotic fluid and elevated levels in pregnant women suggest involvement in gestation and embryonic development. GDF-15 generally exhibits tumor suppressive activities and is secreted in response to activation of the p53 pathway. Serum levels of GDF-15 correlate with progression of several tumor types, including colorectal, pancreatic, and prostate cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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