Thermo Fisher Scientific IL-4 Polyclonal Antibody, HRP
인간 IL-4에 반응하는 HRP 결합 rabbit polyclonal 항체로, WB, IHC, ELISA에 적합합니다. 정제된 항체는 lyophilized 형태이며, 장기 보관 시 -20°C에서 안정적입니다. 면역조절성 IL-4 단백질 인식에 최적화된 연구용 시약입니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length recombinant human IL-4 protein. |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
- This purified antibody was heated to 56°C for 30 minutes.
- Recognizes both native and recombinant human IL-4 in cell supernatants and certain body fluids.
- A control of similarly diluted normal rabbit IgG is recommended.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and serves as a ligand for the interleukin 4 receptor. IL-4 has a broad range of actions and shares overlapping functions with IL-13 due to receptor cross-binding. STAT6 plays an important role in mediating IL-4 immune regulatory signaling. The IL-4 gene is located within a cytokine gene cluster on chromosome 5q, near IL-13 and IL-5, and is coordinated by long-range regulatory elements over a 120 kb range. IL-4 delta 2 is a known splice variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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