Thermo Fisher Scientific IL-33 Polyclonal Antibody, HRP
인간 IL-33 단백질을 인식하는 HRP 결합 폴리클로날 항체로, Western blot, IHC, ELISA 등 다양한 면역분석에 적합합니다. 고순도 정제 및 안정적 보관이 가능하며, 연구용으로만 사용됩니다.
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Thermo Fisher Scientific IL-33 Polyclonal Antibody, HRP
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified antibody prepared from whole rabbit serum produced by repeated immunizations with full-length recombinant human IL-33 protein |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Storage Buffer | 0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid; dilute only prior to immediate use.
- In ELISA and other immunoreactive assays, recognizes both native and recombinant human IL-33 in cell supernatants and certain body fluids.
- A control of similarly diluted normal rabbit IgG is recommended.
Target Information
IL-33 (Interleukin-33) is a 270 amino acid protein belonging to the IL-1 family. It functions both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties.
IL-33 binds and signals through IL1RL1/ST2, recruiting MYD88, IRAK1, IRAK4, and TRAF6, leading to activation of NF-kappaB and MAP kinases. It induces TH2-associated cytokines (IL-4, IL-5, IL-13) and contributes to pathological changes in mucosal organs.
Highly expressed in high endothelial venules of tonsils, Peyer’s patches, and mesenteric lymph nodes; nearly undetectable in placenta.
In mice, prolonged IL-33 treatment causes eosinophilia, splenomegaly, and mucosal organ pathology.
Despite its predicted molecular weight, IL-33 often migrates at higher molecular weight in SDS-PAGE.
IL-33 can co-localize with heterochromatin and act as a transcriptional repressor, reinforcing its dual role as cytokine and nuclear factor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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