Thermo Fisher Scientific IL-3 Polyclonal Antibody, HRP
HRP 결합형 Anti-Human IL-3 폴리클로날 항체로, WB, IHC, ELISA 등 다양한 면역분석에 적합합니다. 정제된 Rabbit IgG로 제조되었으며, 인간 IL-3의 native 및 recombinant 형태를 모두 인식합니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-Human IL-3 (Rabbit) Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full-length recombinant human IL-3 protein. |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
- Purified IgG fraction antibody obtained from monospecific antiserum by delipidation, salt fractionation, ion exchange chromatography, and dialysis.
- Heated to 56°C for 30 minutes for stability.
- Recognizes both native and recombinant human IL-3 in cell supernatants and certain body fluids.
- Recommended to use similarly diluted normal rabbit IgG as a control.
Target Information
Interleukin 3 (IL-3), also known as Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells, and eosinophils.
IL-3 is a hematopoietic growth factor stimulating colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell, and monocytic lineages.
Functions are enhanced or dependent on co-stimulation with other cytokines.
Human and mouse IL-3 share only 29% sequence identity, and bioactivity is species-specific — human IL-3 has no activity on mouse cells.
IL-3 supports proliferation of diverse hematopoietic cell types and is involved in cell growth, differentiation, and apoptosis. It also possesses neurotrophic activity and may be associated with neurologic disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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