Thermo Fisher Scientific MIP-3 alpha Polyclonal Antibody, HRP
Human MIP-3 alpha(also known as CCL20)에 특이적인 HRP 결합 폴리클로날 항체로, WB, IHC, ELISA 등 다양한 분석에 사용 가능. Protein A로 정제된 고순도 제품이며, 장기 보관 시 -20°C에서 안정적. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:20,000–1:100,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MIP-3 alpha Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-3alpha protein. |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
In ELISA and other immunoreactive assays, this antibody recognizes both native and recombinant human MIP-3 alpha in cell supernatants and certain body fluids.
A control of similarly diluted normal rabbit IgG is recommended.
Target Information
Macrophage Inflammatory Protein-3 alpha (MIP-3alpha), also called CCL20, is expressed in the liver, lungs, lymph nodes, and peripheral blood lymphocytes.
MIP-3alpha is strongly upregulated by inflammatory signals and downregulated by the anti-inflammatory cytokine IL-10.
MIP-3alpha signals through the G protein-coupled receptor CCR6 and acts as a chemoattractant to lymphocytes and dendritic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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