Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), APC, eBioscience
Mouse CD163를 인식하는 TNKUPJ 클론 단일클론 항체로, APC 형광표지되어 있습니다. Flow cytometry 및 IHC에 적합하며, 0.25 µg/test 권장 농도로 사용됩니다. 조직 대식세포 표지에 유용하며 4°C 암소 보관이 필요합니다.
- 카탈로그번호
- 17-1631-8x (2개 옵션)
- 판매단위
- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), APC, eBioscience
Applications
Immunohistochemistry (IHC)
- Tested Dilution: Not specified
- View 1 publication
Flow Cytometry (Flow)
- Tested Dilution: 0.25 µg/test
- View 16 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TNKUPJ |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2784645 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 532
- Brilliant UV 615
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- eFluor 450
- FITC
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
This TNKUPJ monoclonal antibody recognizes mouse CD163, a 130 kDa surface receptor expressed by subsets of tissue macrophages such as splenic red pulp macrophages, Kupffer cells, intestinal lamina propria macrophages, and a small fraction of peritoneal macrophages. Mouse monocytes do not express CD163, and unlike human CD163, it is not a strong M2 macrophage marker.
This antibody can detect intracellular CD163 in fixed and permeabilized cells. Since aggressive tissue dissociation may reduce surface CD163, intracellular staining is recommended in such cases.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometric analysis of mouse splenocytes
- Recommended use: ≤ 0.25 µg/test (100 µL final volume)
- Cell number range: 10⁵–10⁸ cells/test
- Optimize antibody titration for best performance
- Excitation: 633–647 nm
- Emission: 660 nm
- Laser: Red laser
Target Information
CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein belonging to the scavenger receptor cysteine-rich superfamily and serves as a receptor for the hemoglobin-haptoglobin complex. It protects tissues from oxidative damage and participates in iron recycling via endocytosis of hemoglobin/haptoglobin complexes.
CD163 is expressed on human monocytes and macrophages, upregulated by IL-10, IL-6, and dexamethasone, and shed from the cell surface upon LPS or PMA stimulation. It binds hemoglobin/haptoglobin complexes in a calcium- and pH-dependent manner and triggers intracellular signaling involving calcium mobilization and cytokine secretion (IL-6, CSF1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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