Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), APC, eBioscience

Applications

Immunohistochemistry (IHC)

• Tested Dilution: Not specified
• View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 0.25 µg/test
• View 16 publications

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 532
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

This TNKUPJ monoclonal antibody recognizes mouse CD163, a 130 kDa surface receptor expressed by subsets of tissue macrophages such as splenic red pulp macrophages, Kupffer cells, intestinal lamina propria macrophages, and a small fraction of peritoneal macrophages. Mouse monocytes do not express CD163, and unlike human CD163, it is not a strong M2 macrophage marker.

This antibody can detect intracellular CD163 in fixed and permeabilized cells. Since aggressive tissue dissociation may reduce surface CD163, intracellular staining is recommended in such cases.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of mouse splenocytes
  • Recommended use: ≤ 0.25 µg/test (100 µL final volume)
  • Cell number range: 10⁵–10⁸ cells/test
  • Optimize antibody titration for best performance
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red laser

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein belonging to the scavenger receptor cysteine-rich superfamily and serves as a receptor for the hemoglobin-haptoglobin complex. It protects tissues from oxidative damage and participates in iron recycling via endocytosis of hemoglobin/haptoglobin complexes.

CD163 is expressed on human monocytes and macrophages, upregulated by IL-10, IL-6, and dexamethasone, and shed from the cell surface upon LPS or PMA stimulation. It binds hemoglobin/haptoglobin complexes in a calcium- and pH-dependent manner and triggers intracellular signaling involving calcium mobilization and cytokine secretion (IL-6, CSF1).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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