Thermo Fisher Scientific CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), APC, eBioscience
인간 CD163 단백질을 표적하는 APC 결합 단클론 항체로, 흐름세포분석(Flow Cytometry)에 최적화됨. 혈액 및 조직 내 대식세포 표지에 활용 가능. 고순도 친화 크로마토그래피 정제, 4°C 암소 보관.
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Thermo Fisher Scientific CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), APC, eBioscience™
Applications and Tested Dilution
- Application: Flow Cytometry
- Tested Dilution: 5 µL (0.5 µg)/test
- Publications: View 31 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioGHI/61 (GHI/61) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573167 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- Biotin
- Brilliant Violet 421
- Brilliant Violet 711
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The eBioGHI/61 monoclonal antibody reacts with human CD163 (Haemoglobin Scavenger Receptor, M130), a member of the macrophage scavenger receptor family. CD163 is expressed by tissue macrophages, circulating blood monocytes, and possibly hematopoietic progenitor cells. It functions as a high affinity scavenger receptor for the hemoglobin-haptoglobin complex, facilitating clearance of hemoglobin from blood.
Upon ligand binding, CD163 triggers signal transduction leading to IL-6 and IL-10 production. Cytokines such as IL-6 and IL-10 can induce CD163 expression, while proinflammatory stimuli like LPS or TNF-α cause shedding of soluble CD163.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and tested on normal human peripheral blood cells
- Recommended Usage: 5 µL (0.5 µg)/test (100 µL final volume)
- Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein and receptor for the hemoglobin-haptoglobin complex. It protects tissues from hemoglobin-mediated oxidative damage and participates in iron recycling through endocytosis and heme breakdown.
CD163 is expressed on human monocytes and macrophages, especially in late inflammation phases. It is upregulated by IL-10, IL-6, and dexamethasone, and shed from the cell surface upon stimulation with LPS or PMA. Binding of hemoglobin/haptoglobin complexes is calcium- and pH-dependent, with higher affinity for multimeric haptoglobin complexes (HP1F phenotype).
CD163 signaling involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and secretion of IL6 and CSF1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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