Thermo Fisher Scientific MAPT Monoclonal Antibody (OTI13E11), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Tau (MAPT) is a neuronal microtubule-associated protein primarily located on axons. It promotes tubulin polymerization and stabilizes microtubules.

• The C-terminus binds axonal microtubules, while the N-terminus associates with neural plasma membrane components, functioning as a linker between both.
• Tau localization determines axonal polarity in neurons.
• Short isoforms contribute to cytoskeletal plasticity, while longer isoforms aid stabilization.
• Hyperphosphorylated Tau forms paired helical filaments (PHF), the major component of neurofibrillary lesions in Alzheimer’s disease brain.
• Hyperphosphorylation disrupts microtubule binding, leading to neuronal degeneration.
• Kinases involved include GSK-3β, PKA, CDK5, and casein kinase II.
• Abnormal Tau phosphorylation is also observed in Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.