Thermo Fisher Scientific NCF4 Monoclonal Antibody (OTI3E3), TrueMAB
Human, Mouse, Rat 반응성의 NCF4 단클론 항체로 Western blot에 최적화됨. Lyophilized 형태로 제공되며 PBS buffer에 trehalose 포함. 재구성 및 conjugation 실험에 적합한 고순도 Affinity purified 제품.
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Applications
- Western Blot (WB): Tested Dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E3 |
| Immunogen | Full length human recombinant protein of human NCF4 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The NCF4 protein is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase complex, essential for host defense.
It is predominantly expressed in myeloid lineage cells and interacts with NCF2/p67-phox and NCF1/p47-phox to form a complex that associates with RAC1 and activates flavocytochrome b upon stimulation.
Its PX domain binds phospholipid products of PI(3) kinase, indicating a role in PI(3) kinase-mediated signaling. Phosphorylation negatively regulates enzyme activity, and multiple alternatively spliced transcript variants exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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