Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (14D3), PE-Cyanine7, eBioscience
인간 및 붉은털원숭이 CD152(CTLA-4)를 인식하는 14D3 단클론 항체입니다. PE-Cyanine7으로 표지되어 유세포 분석에 적합하며, 세포 내 염색 및 활성화된 T세포 연구에 활용됩니다. 광민감성이 있어 암실 보관이 필요합니다.
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Applications
Tested Application
- Flow Cytometry (Flow)
Tested Dilution
- 5 µL (0.06 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rhesus monkey |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14D3 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573405 |
Additional Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 737
- Brilliant UV 805
- eFluor 660
- FITC
- Functional Grade
- PE
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The 14D3 monoclonal antibody reacts with human CD152, also known as cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, structurally similar to CD28, is expressed on activated T cells and binds B7 family members (CD80 and CD86) with higher affinity than CD28. CTLA-4 and CD28 deliver opposing signals: CD28 acts as a costimulator, while CTLA-4 inhibits T cell activation by reducing IL-2 secretion and proliferation.
The 14D3 antibody also recognizes rhesus monkey CTLA-4 and exhibits inhibitory activity.
Applications Reported:
- Intracellular staining followed by flow cytometric analysis.
Applications Tested:
- Tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
For complete detection of CTLA-4, intracellular expression should be assessed in addition to surface expression.
Light Sensitivity:
- The tandem dye is sensitive to photo-induced oxidation; protect from light.
Fixation:
- Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.
Excitation / Emission / Laser:
- 488–561 nm excitation; 775 nm emission; compatible with Blue, Green, and Yellow-Green lasers.
Filtration:
- 0.2 µm post-manufacturing filtered.
Target Information
CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor mainly expressed on CD4+ T cells. It binds CD80 and CD86 with higher affinity than CD28 and inhibits T cell activation. Intracellular CTLA4 is also found in regulatory T cells and plays a role in their function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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