Thermo Fisher Scientific CD154 (CD40 Ligand) Monoclonal Antibody (MR1), PE-Cyanine7, eBioscience
마우스 CD154(CD40 Ligand)을 인식하는 MR1 단클론 항체로, PE-Cyanine7으로 표지되어 있습니다. 활성화된 T세포의 CD154 발현 분석에 적합하며, 유세포분석(Flow Cytometry)에 최적화되어 있습니다. 0.25 µg/test 권장, 4°C 암소 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.25 µg/test | View 2 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MR1 |
| Conjugate | PE-Cyanine7 |
| Excitation/Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573408 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- Biotin
- eFluor 450
- Functional Grade
- PE
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
The MR1 monoclonal antibody reacts with mouse CD154 (CD40 Ligand), a 39 kDa transmembrane glycoprotein transiently expressed by activated T cells.
CD154 binds to CD40 on antigen-presenting cells (B cells, monocytes/macrophages, dendritic cells) and plays a key role in T cell–APC interaction, promoting T-dependent B cell activation and proliferation.
- Application Reported: Flow cytometric analysis
- Application Tested: Flow cytometry of stimulated mouse splenocytes (≤0.25 µg/test)
- One test = antibody amount for 100 µL cell suspension (10⁵–10⁸ cells/test)
- Optimize antibody titration for best results
Light Sensitivity: PE-Cyanine7 is light-sensitive. Protect from light during storage and staining.
Fixation: Samples may be stored up to 3 days at 4°C in the dark using:
- IC Fixation Buffer (Product # 00-8222-49)
- 1-step Fix/Lyse Solution (Product # 00-5333-54)
Excitation/Emission: 488–561 nm / 775 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD40 Ligand (CD40-L, CD154) is a membrane glycoprotein expressed on activated T cells. It stimulates B cell proliferation and immunoglobulin secretion in the presence of cytokines, induces cytokine production and tumoricidal activity in monocytes, and co-stimulates activated T cells to produce IFN-γ, TNF-α, and IL-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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