Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), PE-Cyanine7, eBioscience
마우스 CD152(CTLA-4)를 인식하는 UC10-4B9 단클론 항체로, PE-Cyanine7 형광표지된 Flow Cytometry용 시약입니다. 활성화된 T세포의 CTLA-4 발현 분석에 적합하며, 세포 내 염색에도 사용 가능합니다. 광민감성이 있어 빛을 차단해 보관해야 합니다.
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Applications and Tested Dilution
- Flow Cytometry (Flow): 0.5 µg/test
- Publications: View 11 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UC10-4B9 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2688104 |
Available Formats:
- Unconjugated
- APC
- Biotin
- Brilliant UV 737
- PE
- PE-eFluor 610
- Custom conjugation available
Product Specific Information
The UC10-4B9 monoclonal antibody reacts with mouse CD152 (CTLA-4, cytotoxic T lymphocyte antigen-4). CTLA-4 is structurally similar to CD28 and expressed on activated T cells. It binds B7 family members CD80 (B7-1) and CD86 (B7-2) with higher affinity than CD28, delivering inhibitory signals that restrict T cell activation by reducing IL-2 secretion and proliferation.
Due to its intracellular localization, detection of CTLA-4 may require both surface and intracellular staining.
Applications Reported
- Flow cytometric analysis
- Intracellular staining followed by flow cytometric analysis
Applications Tested
Tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the FoxP3/Transcription Factor Staining Buffer Set (Product #00-5523-00). Recommended usage ≤ 0.5 µg per test (100 µL final volume). Optimal titration required.
Handling and Storage
- Light sensitivity: Protect from light to prevent photo-induced oxidation.
- Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark.
Fluorophore Information
- Excitation: 488–561 nm
- Emission: 775 nm
- Compatible Lasers: Blue, Green, Yellow-Green
Target Information
CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor mainly expressed on CD4+ T cells. It binds CD80 and CD86 with higher affinity than CD28, delivering inhibitory signals that suppress T cell activation. Intracellular CTLA4 is also found in regulatory T cells and contributes to their immunosuppressive functions.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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