Thermo Fisher Scientific Phospho-MAPRE3 (Ser176) Polyclonal Antibody
Phospho-EB3(Ser176)을 인식하는 다클론 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot에 최적화되어 있으며, 항원 친화 크로마토그래피로 정제되었습니다. -20°C에서 보관하며, 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-EB3 (Ser-176) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 176 in human EB3. Highly conserved in mouse and rat EB3. Conserved site in EB2, but neighboring amino acids not well conserved. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942913 |
Product Specific Information
This antibody was cross-absorbed to unphospho-EB3 (Ser-176) before affinity purification using phospho-EB3 (Ser-176) peptide (without carrier). The antibody detects a 32 kDa protein corresponding to the apparent molecular mass of phosphorylated EB3 on SDS-PAGE immunoblots of mouse brain. This reactivity is significantly reduced after lambda phosphatase treatment.
Target Information
EB3 is a 282 amino acid homologue of EB1, a protein known to bind APC (adenomatous polyposis coli). It binds to tubulin in vitro and microtubules in vivo. Expression is mainly in brain, with lower levels in muscle. EB3 interacts with APCL, a homologue of the tumor suppressor APC, and this interaction may be involved in the stabilization or extension of microtubules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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