Thermo Fisher Scientific Phospho-EEF2K (Ser359) Polyclonal Antibody

Applications and Tested Dilution

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-eEF2K (Ser-359) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-359 in human eEF2K.
This sequence is well conserved in rat and mouse eEF2K and has low homology to other proteins.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage Buffer

PBS with 1 mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage Conditions

-20°C, avoid freeze/thaw cycles

Shipping Conditions

Wet ice

RRID

AB_2942916

Product Specific Information

The antibody detects a 105 kDa protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of human recombinant eEF2K autophosphorylated in the presence of Ca²⁺ and Calmodulin.
This reactivity is removed after lambda phosphatase treatment.

Target Information

EEF2K (Eukaryotic Elongation Factor 2 Kinase) is a highly conserved serine/threonine kinase in the calmodulin signaling pathway.
Its activity is increased in cancers including breast cancer.
EEF2K regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by upstream kinases such as AMPK or TRPM7, EEF2K phosphorylates elongation factor EEF2 at a single site, rendering EEF2 unable to bind ribosomes and reducing the rate of protein synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.