Thermo Fisher Scientific PLA2R1 Monoclonal Antibody (CL0474)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:20,000-1:50,000

-

Immunocytochemistry (ICC/IF)

2-10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

CL0474

Immunogen

Recombinant protein corresponding to Human PLA2R1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PLA2R1,uniProtId:Q13018-1,ncbiNodeId:9606,antigenRange:395-530,antigenLength:1463,antigenImageFileName:MA5-24607_PLA2R1_Q13018-1_House_mouse.svg,antigenImageFileNamePDP:MA5-24607_PLA2R1_Q13018-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2637222

Product Specific Information

Immunogen sequence: EEKTWHEALR SCQADNSALI DITSLAEVEF LVTLLGDENA SETWIGLSSN KIPVSFEWSN DSSVIFTNWH TLEPHIFPNR SQLCVSAEQS EGHWKVKNCE ERLFYICKKA GHVLSDAESG CQEGWERHGG FCYKID

Highest antigen sequence identity to the following orthologs: Mouse - 74%, Rat - 76%.

Binds to an epitope located within the peptide sequence KIPVSFEWSN as determined by overlapping synthetic peptides.

Target Information

Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.