Thermo Fisher Scientific REST Monoclonal Antibody (CL0381)

Applications

Tested Dilution

Publications

Western Blot (WB)

1.4 µg/mL

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2-10 µg/ml

-

ChIP assay (ChIP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

CL0381

Immunogen

Recombinant protein corresponding to Human REST if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:REST,uniProtId:Q13127-1,ncbiNodeId:9606,antigenRange:864-991,antigenLength:1097,antigenImageFileName:MA5-24606_REST_Q13127-1_House_mouse.svg,antigenImageFileNamePDP:MA5-24606_REST_Q13127-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.7 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2637221

Product Specific Information

Immunogen sequence: SPPLPKENLR EEASGDQKLL NTGEGNKEAP LQKVGAEEAD ESLPGLAANI NESTHISSSG QNLNTPEGET LNGKHQTDSI VCEMKMDTDQ NTRENLTGIN STVEEPVSPM LPPSAVEERE AVSKTALA

Highest antigen sequence identity to the following orthologs: Mouse - 41%, Rat - 43%.

Binds to an epitope located within the peptide sequence ISSSGQNLNT as determined by overlapping synthetic peptides.

Target Information

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.