Thermo Fisher Scientific REST Monoclonal Antibody (CL0381)
REST 단백질을 인식하는 Mouse monoclonal antibody (Clone CL0381). Western blot, IHC, ICC/IF, ChIP 등 다양한 응용에 사용 가능. Human에 반응하며 Protein A로 정제된 액상형 항체. 장기 보관은 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1.4 µg/mL | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | 1 publication |
| Immunocytochemistry (ICC/IF) | 2–10 µg/mL | - |
| ChIP assay (ChIP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CL0381 |
| Immunogen | Recombinant protein corresponding to Human REST (aa 864–991, UniProt ID: Q13127-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.7 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2637221 |
Product Specific Information
Immunogen sequence:
SPPLPKENLR EEASGDQKLL NTGEGNKEAP LQKVGAEEAD ESLPGLAANI NESTHISSSG QNLNTPEGET LNGKHQTDSI VCEMKMDTDQ NTRENLTGIN STVEEPVSPM LPPSAVEERE AVSKTALA
- Highest antigen sequence identity to orthologs: Mouse – 41%, Rat – 43%
- Binds to an epitope located within the peptide sequence ISSSGQNLNT as determined by overlapping synthetic peptides.
Target Information
Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites, acting as a chromatin modifier through histone deacetylation and demethylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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