Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Brilliant Violet 480, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor series (450, 506, 660)
• FITC, PE, PE-Cyanine series
• PerCP-Cyanine5.5
• Super Bright series (436–780)
• NovaFluor series (Blue, Red, Violet, Yellow variants)
• Functional Grade
• Request custom conjugation available

Product Specific Information

The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all stages of development except terminal plasma cells. Follicular dendritic cells also express CD19.
CD19 forms a multimolecular complex with CD21, CD81, and MHC class II, associating with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and B cell proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes, ≤1.0 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Optimization: Titrate for best performance

Fluorochrome Information:
Brilliant Violet™ 480 emits at 479 nm, suitable for cytometers with a 405 nm violet laser. Ensure instrument compatibility.
Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) when combining polymer dye-conjugated antibodies to minimize non-specific interactions.
Not compatible with methanol-based fixation.
Excitation: 440 nm; Emission: 479 nm; Laser: Violet.

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. Expressed on all peripheral B cells and certain myeloid leukemia cells.
It serves as a critical regulator of normal and malignant B cell proliferation and is expressed in all B cell precursor leukemias.
CD19 acts as a signal-amplifying coreceptor for the B cell receptor (BCR) and can also signal independently.
Mutations lead to hypogammaglobulinemia; overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures.

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