Thermo Fisher Scientific CD38 Monoclonal Antibody (90), Brilliant Violet 480, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant Violet 605
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The 90 monoclonal antibody reacts with the mouse CD38 molecule (~42 kDa type II transmembrane protein). CD38 expression increases during B-cell differentiation, is down-regulated on germinal center B cells and plasma cells, and is present on subsets of thymocytes, T cells, and NK cells.
Crosslinking of CD38 on mature B cells induces proliferation, enhanced by IL-4 and LPS. CD38, a counter-receptor for CD31, functions as an ectoenzyme with cyclase and hydrolase activity, involved in lymphocyte activation and differentiation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤0.5 µg/test in 100 µL final volume. Recommended to titrate for optimal performance.

Fluorochrome Information:
Brilliant Violet™ 480 (BV480) emits at 479 nm, suitable for cytometers with a 405 nm violet laser.
Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) when combining polymer dye-conjugated antibodies to minimize non-specific interactions.
BV480 is not compatible with methanol-based fixation.

• Excitation: 440 nm
• Emission: 479 nm
• Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD38 (NAD⁺ glycohydrolase) is a type II transmembrane glycoprotein involved in lymphocyte activation, proliferation, and differentiation, as well as apoptosis of progenitor cells. It functions as an ectoenzyme (ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase, NAD⁺ glycohydrolase) and as a receptor.
Antibodies to CD38 are used for subtyping lymphomas/leukemias, plasma cell detection (e.g., myeloma identification), and as markers for activated B and T cells.
CD38 participates in cell adhesion, signal transduction, and calcium signaling, and is highly expressed in pancreas, liver, kidney, malignant lymphoma, and neuroblastoma.
Associated diseases include chronic lymphocytic leukemia and Richter’s Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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