Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Brilliant Violet 480, eBioscience
CD16(FcγRIII)를 인식하는 eBioCB16 단클론 항체로, 인간 NK세포 및 대식세포의 CD16 검출에 적합. Brilliant Violet 480 형광체로 결합되어 405nm 레이저 기반 유세포분석에 최적. 메탄올 고정 불가, 연구용 전용.
- 카탈로그번호
- 414-0168-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Brilliant Violet 480, eBioscience
Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Brilliant Violet 480, eBioscience
Applications
- Flow Cytometry (Flow): 5 µL (0.5 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet™ 480 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioCB16 (CB16) |
| Conjugate | Brilliant Violet™ 480 |
| Excitation/Emission Max | 437 / 478 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2929089 |
Additional Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661 / 737 / 805
- eFluor 450 / 506
- FITC
- Functional Grade
- NovaFluor Blue 610-70S / 660-120S
- NovaFluor Red 710 / 725
- NovaFluor Yellow 660 / 730
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG (50–80 kDa).
CD16 includes two genes: CD16A (FcγRIIIA) expressed in macrophages and NK cells, and CD16B (FcγRIIIB) expressed in neutrophils.
Two polymorphisms of CD16B (NA-1, NA-2) affect phagocytic capacity.
CD16 binds IgG immune complexes, showing preferential binding to IgG1 and IgG3. Upon binding, CD16 initiates signal transduction leading to ADCC, phagocytosis, degranulation, and proliferation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-diluted, tested on normal human peripheral blood cells. Use at 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
- Fluorochrome: Brilliant Violet™ 480 emits at 479 nm, suitable for 405 nm laser detection.
- Compatibility Note: Not compatible with methanol-based fixation.
- Recommended Buffers: Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions.
- Excitation/Emission/Laser: 440 nm / 479 nm / Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
CD16 encodes a receptor recognizing the Fc portion of IgG, involved in immune complex clearance and cell-mediated cytotoxicity.
The FCGR3A gene (chromosome 1) is expressed in NK cells and macrophages, while FCGR3B is expressed in neutrophils.
Mutations in FCGR3A are associated with Immunodeficiency 20, Herpes Zoster, and systemic lupus erythematosus susceptibility.
Alternative splicing yields multiple isoforms.
For Research Use Only. Not for use in diagnostic procedures.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.