Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Brilliant Violet 480, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661 / 737 / 805
• eFluor 450 / 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S / 660-120S
• NovaFluor Red 710 / 725
• NovaFluor Yellow 660 / 730
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG (50–80 kDa).
CD16 includes two genes: CD16A (FcγRIIIA) expressed in macrophages and NK cells, and CD16B (FcγRIIIB) expressed in neutrophils.
Two polymorphisms of CD16B (NA-1, NA-2) affect phagocytic capacity.
CD16 binds IgG immune complexes, showing preferential binding to IgG1 and IgG3. Upon binding, CD16 initiates signal transduction leading to ADCC, phagocytosis, degranulation, and proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted, tested on normal human peripheral blood cells. Use at 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
• Fluorochrome: Brilliant Violet™ 480 emits at 479 nm, suitable for 405 nm laser detection.
• Compatibility Note: Not compatible with methanol-based fixation.
• Recommended Buffers: Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions.
• Excitation/Emission/Laser: 440 nm / 479 nm / Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD16 encodes a receptor recognizing the Fc portion of IgG, involved in immune complex clearance and cell-mediated cytotoxicity.
The FCGR3A gene (chromosome 1) is expressed in NK cells and macrophages, while FCGR3B is expressed in neutrophils.
Mutations in FCGR3A are associated with Immunodeficiency 20, Herpes Zoster, and systemic lupus erythematosus susceptibility.
Alternative splicing yields multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures.

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