Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), FITC, eBioscience
마우스 TCR V alpha 2 사슬을 인식하는 B20.1 단클론 항체로, FITC 형광 표지되어 있습니다. Flow Cytometry에 최적화되어 있으며, 0.25 µg/test로 사용 가능합니다. PBS 완충액에 보관하며, 4°C 암소 보관이 권장됩니다.
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- 11-5812-8x (2개 옵션)
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카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), FITC, eBioscience
Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 5 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, lambda |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B20.1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465258 |
Available Conjugates
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- NovaFluor Blue 610-70S
- NovaFluor Red 700
- NovaFluor Yellow 570
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 780
- Request custom conjugation
Product Specific Information
Description:
The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains. It recognizes the majority of the TCR V alpha 2 subfamily in mice carrying the a, b, and c haplotypes, and also reacts with TCR V delta 8 products due to high homology.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse lymph node cells. Recommended ≤ 0.25 µg/test.
A test is defined as the amount of antibody that stains a cell sample in 100 µL final volume (typically 10⁵–10⁸ cells/test).
Preblock Fc receptors for 10 minutes using 0.5 µg purified anti-mouse CD16/32 (Product #14-0161-85).
Careful titration is recommended for optimal performance.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
T cell receptors (TCR) are essential for distinguishing foreign from self-peptides presented by MHC class II molecules, critical for adaptive immunity.
Recognition of self-peptides is linked to autoimmune diseases, while mutant self-peptides are associated with tumors.
Superantigen engagement of TCRs can cause toxic shock syndrome.
Autoantibodies to V beta TCR segments have been identified in rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), potentially modulating inflammatory responses.
Most human T cells express TCR alpha-beta with CD4 or CD8, while a subset (double negative) lacks both. Increased alpha-beta DN T cells are associated with autoimmune and immunodeficiency disorders. Gamma-delta T cells, found mainly in epithelia, exhibit limited diversity and distinct antigen recognition, with roles in antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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