Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), FITC, eBioscience
CD326(EpCAM) 인식 단일클론 항체(G8.8)로, FITC 형광 표지되어 Flow Cytometry 등 다양한 면역분석에 적합. 마우스 반응성, Rat IgG2a 아이소타입. 0.5 µg/test 권장, 4°C 보관. EpCAM 발현 세포 연구 및 암 관련 면역학 연구에 활용 가능.
- 카탈로그번호
- 11-5791-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 13 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 2 |
| Immunocytochemistry (ICC/IF) | - | 15 |
| Flow Cytometry (Flow) | 0.5 µg/test | 68 |
| Affinity Purification (AP) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G8.8 |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151327 |
Additional Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 450
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation available
Product Specific Information
The G8.8 monoclonal antibody reacts with the 40 kDa protein mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40, 17-1A antigen, TACSTD1, and CD326. The immunogen used was the TE-71 thymic epithelial cell line. CD326 is expressed on most epithelial cells and is considered a pan-carcinoma antigen mediating calcium-independent, homophilic cell-cell adhesion and possibly acting as a growth factor receptor.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Validated by flow cytometric analysis of TE-71 cells at ≤0.5 µg/test.
A test is defined as the amount of antibody staining a cell sample in 100 µL volume.
Cell number range: 10⁵–10⁸ cells/test.
Optimal titration recommended for best assay performance.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent adhesion molecule influencing cell cycle, proliferation, and metabolism via c-myc and cyclin A/E regulation. Ep-CAM overexpression correlates with enhanced epithelial proliferation and carcinoma marker expression. It is localized on the basolateral surface of most simple epithelia and distinguishes adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.