Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation available

Product Specific Information

The G8.8 monoclonal antibody reacts with the 40 kDa protein mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40, 17-1A antigen, TACSTD1, and CD326. The immunogen used was the TE-71 thymic epithelial cell line. CD326 is expressed on most epithelial cells and is considered a pan-carcinoma antigen mediating calcium-independent, homophilic cell-cell adhesion and possibly acting as a growth factor receptor.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Validated by flow cytometric analysis of TE-71 cells at ≤0.5 µg/test.
A test is defined as the amount of antibody staining a cell sample in 100 µL volume.
Cell number range: 10⁵–10⁸ cells/test.
Optimal titration recommended for best assay performance.

Excitation: 488 nm
Emission: 520 nm
Laser: Blue
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent adhesion molecule influencing cell cycle, proliferation, and metabolism via c-myc and cyclin A/E regulation. Ep-CAM overexpression correlates with enhanced epithelial proliferation and carcinoma marker expression. It is localized on the basolateral surface of most simple epithelia and distinguishes adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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