Thermo Fisher Scientific TCR V beta 8.1/8.2 Monoclonal Antibody (KJ16-133 (KJ-16, KJI6, KJ-I6)), FITC, eBioscience

Thermo Fisher Scientific TCR V beta 8.1/8.2 Monoclonal Antibody (KJ16-133), FITC

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test
• View 4 publications

Product Specifications

Additional Formats

• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This KJ16-133 monoclonal antibody recognizes mouse T cell receptors (TCR) containing Vβ8.1 or 8.2, expressed by approximately 15–20% of peripheral T cells in most mouse strains (e.g., Balb/c, AKR, C57BL/6, B10.A) and several T cell hybridomas (e.g., DO-11.10, 3DT-52.5, D1G10G11, SKK-9.11, SKK-45.10, MDK16, S18.4).

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of mouse splenocytes
  • Use ≤ 0.25 µg per test (100 µL final volume, 10⁵–10⁸ cells/test)
  • Optimize titer for best performance

Fluorescence Characteristics:

• Excitation: 488 nm
• Emission: 520 nm
• Laser: Blue
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

T cell receptors (TCR) discriminate between self and foreign peptides presented by MHC class II molecules, a critical process for adaptive immunity. Recognition of self-peptides is linked to autoimmune diseases, while mutant self-peptides are associated with tumors. Superantigen engagement of TCRs can cause toxic shock syndrome. Autoantibodies to TCR Vβ segments have been found in rheumatoid arthritis and systemic lupus erythematosus, potentially modulating immune responses. Most human T cells express TCR αβ with CD4 or CD8, while a minority are double-negative (DN), which can increase in autoimmune or immunodeficiency conditions. γδ T cells, mainly in epithelia, show limited diversity and distinct antigen recognition, with reported antitumor and immunoregulatory roles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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