Thermo Fisher Scientific RPH3A Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

PA1-774 detects rabphilin 3A from rat tissues.
It has been successfully used in Western blot procedures.
By Western blot, this antibody detects an ~75 kDa protein representing rabphilin 3A from rat brain whole protein extract.

The immunogen is a synthetic peptide corresponding to residues M(1)TDTVVNRWMYPGDGPLQ(18)C of rat Rabphilin 3A, a sequence completely conserved between rat and mouse rabphilin 3A.
The immunizing peptide (Cat. # PEP-102) is available for use in neutralization and control experiments.

Target Information

Rabphilin-3A contains two C2 domains (A and B) at the carboxy-terminal and a cysteine-rich region (residues 45–170) with two zinc finger motifs at the amino-terminal.
It belongs to a family of C-type tandem C2 proteins, including synaptotagmins and Doc2.
Rabphilin is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules.
It binds Rab3a/GTP/Mg²⁺ within amino-terminal residues 45–170, and binds calcium ions and phosphatidylinositol 4,5-bisphosphate-containing lipid vesicles within its C2 domains.
Rabphilin-3A acts as a positive regulator of calcium-dependent exocytosis, while Rab3a acts as a negative regulator.
Although they associate, their effects on exocytosis appear to be independent, likely mediated by an unknown factor recognizing the Rab3 binding domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.