Thermo Fisher Scientific RPH3A Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

2 µg/mL

-

Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) T D T V V N R W M Y P G D G P L Q(18) C of rat Rabphilin 3A. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RPH3A,uniProtId:P47709-1,ncbiNodeId:10114,antigenRange:1-18,antigenLength:684,antigenImageFileName:PA1-774_RPH3A_P47709-1_Rabbit.svg,antigenImageFileNamePDP:PA1-774_RPH3A_P47709-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2181152

Product Specific Information

PA1-774 detects rabphilin 3A from rat tissues.

PA1-774 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~75 kDa protein representing rabphilin 3A from rat brain whole protein extract.

PA1-774 immunogen is a synthetic peptide corresponding to residues M(1) T D T V V N R W M Y P G D G P L Q(18) C of rat Rabphilin 3A. This sequence is completely conserved between rat and mouse rabphilin 3A. PA1-774 immunizing peptide (Cat. # PEP-102) is available for use in neutralization and control experiments.

Target Information

The carboxy-terminal of rabphilin-3A consists of two C2 domains, A and B, and the amino-terminal (residues 45-170) contains a cysteine-rich rich region with two zinc finger motifs. Rabphilin-3A belongs to a family of other carboxyterminal type (C-type) tandem C2 proteins, which includes synaptotagmins and Doc2. Rabphilin is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules. Rabphilin-3A binds Rab3a/GTP/Mg+ within amino-terminal residues 45 and 170. Rabphilin-3A binds calcium ions and phosphatidylinositol 4,5-bisphophate containing lipid vesicles within its C2 domains. Rabphilin-3A is a positive regulator of calcium dependent exocytosis, while Rab3a is a negative regulator of exocytosis. Although rabphilin-3A associates with Rab3a, they seem to influence exocytosis independently of each other. Rabphilin-3A effects are likely mediated through interactions with an unknown factor that recognizes the Rab3 binding domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.