Thermo Fisher Scientific VAMP4 Polyclonal Antibody
VAMP4 단백질 검출용 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 고순도 항체로 안정적인 신호 제공.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 3 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | View publication |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | View 4 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant rat VAMP-4 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2212790 |
Product Specific Information
PA1-768 detects VAMP-4 from human and mouse samples.
It has been successfully used in Western blot and immunofluorescence procedures.
By Western blot, this antibody detects an ~18 kDa protein representing VAMP-4 from PC3 cell extract.
Immunofluorescent staining of VAMP-4 in CV-1 cells using PA1-768 results in a pattern consistent with VAMP-4 localization.
Immunofluorescence staining with this antibody requires saponin permeabilization.
The PA1-768 antigen is recombinant rat VAMP-4.
Target Information
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium-binding proteins specific to eukaryotes.
VAMPs, along with SNAP-25 and syntaxin, form the core complex of SNARE proteins that interact with NSF and alpha-SNAP.
These membrane-associated proteins play a key role in regulating vesicle membrane fusion with the plasma membrane.
Clostridium tetani neurotoxin acts as a metalloprotease specific for VAMP.
In Alzheimer’s disease, VAMP levels of all isoforms appear to be significantly lowered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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