Thermo Fisher Scientific beta-3 Tubulin Monoclonal Antibody (TU-20)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL, 90 min

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 4 publications 4 publications

Flow Cytometry (Flow)

1-4 µg/mL

-

Product Specifications

Species Reactivity

Human, Many, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

TU-20

Immunogen

Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Beta III Tubulin,uniProtId:Q13509-1,ncbiNodeId:9606,antigenRange:441-448,antigenLength:450,antigenImageFileName:MA1-19187_Beta_III_Tubulin_Q13509-1_House_mouse.svg,antigenImageFileNamePDP:MA1-19187_Beta_III_Tubulin_Q13509-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• FITC
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1070721

Product Specific Information

This antibody recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.

For immunohistochemistry applications, this product requires protein pretreatment (0.1% trypsin) in 0.1 M HCl; incubate for 30 minutes at room temperature.

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

Western Blot: Reducing conditions.

Target Information

The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.