Thermo Fisher Scientific CD22 Monoclonal Antibody (MEM-01)
CD22 단백질을 인식하는 Thermo Fisher Scientific의 MEM-01 단클론 항체. 인간 및 비인간 영장류 반응성, 단백질 A 정제, 액상 형태로 제공. WB, Flow Cytometry, IP에 사용 가능하며 B세포 표지 분석에 적합.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 2–4 µg/mL | 120 min on vertical incubator |
| Flow Cytometry (Flow) | 2–4 µg/mL | — |
| Immunoprecipitation (IP) | Assay-dependent | — |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-01 |
| Immunogen | Burkitt’s lymphoma cell line Raji |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1071663 |
Additional Formats
Product Specific Information
This antibody recognizes uniquely epitope “E”; it does not cross-block antibodies RFB-4, CLB22/1, and CLB-BLy1.
Western Blot: 120 min on vertical incubator.
Target Information
CD22 (BL-CAM) is a type 1 integral membrane glycoprotein (130–140 kDa) expressed in both cytoplasm and cell membrane of B-lymphocytes.
CD22 appears early in B-cell differentiation, similar to CD19 expression. Its membrane expression is limited to mature B cells (CD22+) and plasma cells (CD22−), making it useful for phenotyping mature leukemias.
CD22 is strongly expressed in hairy cell leukemia, diffuse large B-cell lymphoma, and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.
It binds preferentially to α2,6-linked sialic acid and participates in regulation of B-cell antigen receptor signaling through interactions with Src family tyrosine kinases and SH2-domain phosphatases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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