Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Super Bright 600, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor 450, 506, 570, 615
• FITC
• NovaFluor Blue / Yellow series
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 645, 702, 780
• Request custom conjugation

Product Specific Information

The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, subsets of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and is essential for T cell development and function. Binding of RM4-5 is blocked by GK1.5.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes; use ≤0.125 µg/test (100 µL volume, 10⁵–10⁸ cells/test). Optimize concentration for best performance.
• Fluorochrome: Super Bright 600 (excitation 405 nm, emission 600 nm; violet laser compatible). Use 610/20 bandpass filter.
• Buffer Recommendation: When using multiple Super Bright-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize polymer interactions.
• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Samples can be stored up to 3 days at 2–8°C in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) with minimal impact on brightness.
• Laser: Violet Laser (405 nm excitation, 600 nm emission)

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD4 antigen is a co-receptor for MHC class II molecules and is involved in T-cell activation. It is primarily expressed in T helper cells, and also in monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and certain brain and gut regions. CD4 functions to initiate or enhance T-cell activation through association with the T-cell receptor complex and protein tyrosine kinase Lck. It may also play a role in neuronal damage in infectious and immune-mediated CNS diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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