Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Super Bright 600, eBioscience
인간 CD3 복합체의 엡실론 서브유닛을 인식하는 OKT3 단클론 항체. Super Bright 600 형광체 결합으로 유세포분석에 최적. 면역조직화학, 면역세포분석, T세포 활성화 연구에 활용 가능. 405 nm 레이저로 여기, 600 nm에서 방출. 빛에 민감하므로 어둡게 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 2 |
| Immunocytochemistry (ICC/IF) | - | 4 |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | 27 |
| Functional Assay | - | 1 |
| Immunomicroscopy (IM) | - | 1 |
| T-Cell Activation (TCA) | - | 5 |
| Miscellaneous (PubMed) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 600 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT3 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637434 |
Additional Formats Available
Unconjugated, Alexa Fluor 488, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395/563/615/661/737/805, Brilliant Violet 421/605, eFluor 450/506/660, FITC, Functional Grade, NovaFluor Blue 510/585, NovaFluor Yellow 590/660/730, PE, PE-Cyanine7, PE-eFluor 610, PerCP-Cyanine5.5, PerCP-eFluor 710, Super Bright 436/645/702/780, Custom conjugation available.
Product Specific Information
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. It has potent immunosuppressive properties and is effective in treating renal, heart, and liver allograft rejection. CD3 is expressed by thymocytes and all mature T cells, playing a key role in TCR complex assembly and activation.
- Applications Reported: Flow cytometry
- Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells at 5 µL (0.25 µg) per test.
- Laser Excitation: 405 nm (Violet)
- Emission: 600 nm
- Recommended Filter: 610/20 bandpass
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.
Light Sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 2–8°C in the dark.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
The CD3 subunit complex is essential for T-cell receptor (TCR) signal transduction and surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits, all encoded on human chromosome 11. CD3 is expressed on most T lymphocytes and thymocytes but not on B or NK cells. It plays roles in T-cell activation, proliferation, and signal transduction. Defects in CD3 genes are linked to immunodeficiency disorders.
For Research Use Only. Not for use in diagnostic procedures.
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