Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), Super Bright 600, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (1.0 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 737
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• Super Bright 436
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBio124-1D1 monoclonal antibody reacts with human CD7 (gp40, Leu9), a 40 kDa receptor belonging to the immunoglobulin superfamily.

• Expression: Found on immature and mature T lymphocytes, T cell leukemias, NK cells, a subset of normal and malignant B cells.
• Function: Cross-linking CD7 modulates T and NK cell activity, affecting calcium flux, adhesion molecule expression, cytokine secretion, and proliferation.
• Signaling: CD7 associates with phosphoinositol 3′-kinase; ligation induces D-3 phosphoinositide production and tyrosine phosphorylation.
• Stem Cell Relevance: CD7 expression identifies primitive lymphoid progenitors capable of generating B, NK, and dendritic cells but not myeloid or erythroid lineages.
• Treg Function: CD7 may cooperate with CD28; deficiency in both reduces Treg numbers and suppressive function.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted; use 5 µL (1.0 µg) per 100 µL cell sample (10⁵–10⁸ cells/test).

Fluorochrome Information:

• Super Bright 600 is a tandem dye excited at 405 nm, emitting at 600 nm.
• Recommended detection filter: 610/20 bandpass.
• Use Super Bright Complete Staining Buffer (SB-4401) to minimize non-specific polymer interactions.
• Light-sensitive: protect from light.
• Fixation: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C.
• Excitation: 405 nm; Emission: 600 nm; Laser: Violet.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD7 (gp40, Leu9) is a 40 kDa immunoglobulin superfamily member.

• Structure: N-terminal (aa 1–107) homologous to Ig kappa light chain; C-terminal proline-rich region forms a stalk for the Ig domain.
• Expression: Found on immature/mature T cells, T cell leukemia, NK cells, and subsets of B cells.
• Function: Cross-linking modulates T and NK cell activation via PI3K signaling.
• Clinical Relevance: CD7 expression serves as a diagnostic marker in leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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