Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Super Bright 600, eBioscience
마우스 CD4 단백질을 인식하는 GK1.5 클론의 단클론 항체로, Super Bright 600 형광체로 결합되어 플로우 사이토메트리 분석에 최적화됨. 405 nm 레이저로 여기, 600 nm에서 방출. 높은 특이성과 신뢰성으로 면역세포 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 6 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 92 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GK1.5 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2717016 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV/Violet/Yellow series
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Blue/Red/Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 645, 702, 780
- Request custom conjugation
Product Specific Information
The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa surface receptor expressed by thymocytes, subsets of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and is essential for T cell development and function. GK1.5 binding is blocked by RM4-5.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometry of mouse splenocytes at ≤0.125 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Titrate for optimal performance.
Fluorochrome Information:
Super Bright 600 is a tandem dye excited at 405 nm (violet laser) and emits at 600 nm. Recommended detection filter: 610/20 bandpass. Protect from light due to photo-induced oxidation sensitivity.
Staining Recommendations:
When using multiple Super Bright-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal signal loss.
Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD4 is a co-receptor for MHC class II recognition and plays a key role in T-cell activation via association with the T-cell receptor complex and Lck kinase. It is expressed on T helper cells, monocytes, macrophages, and dendritic cells, and is found in thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues. CD4 may also mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts are known (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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