Thermo Fisher Scientific CD85j (ILT2) Monoclonal Antibody (HP-F1), NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Available Formats

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 737
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody HP-F1 recognizes CD85j (ILT2, LILRB1, LIR1), a member of the ILT/LIR/MIR family.
CD85j is a single transmembrane glycoprotein with a long cytoplasmic domain containing four ITIMs that signal through SHP-1.
Expression is found on myeloid cells (monocytes, dendritic cells) and subsets of NK, T, and B cells.
It is correlated with leukemias (ALL, CLL) and viral infections (HIV, EBV, CMV).
Ligands include MHC Class I molecules such as HLA-G, A, F, B27, E, and F.
HP-F1 reduces CD16-dependent cytolytic activity of NK cells.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells.
Recommended use: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample.

NovaFluor Conjugates:
Based on Phiton™ technology utilizing novel nucleic acid dye structures for engineered fluorescent signatures, minimizing spillover and spread.
Excitation: 509 nm; Emission: 614 nm; Laser: 488 nm (Blue)

Target Information

This gene belongs to the leukocyte immunoglobulin-like receptor (LIR) family located at 19q13.4.
It encodes a receptor with extracellular immunoglobulin domains, a transmembrane domain, and cytoplasmic ITIM motifs.
Expressed on immune cells, it binds MHC class I molecules to inhibit immune activation, helping regulate inflammation and cytotoxicity.
Multiple transcript variants exist.

For Research Use Only. Not for use in diagnostic procedures.

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