Thermo Fisher Scientific CD85j (ILT2) Monoclonal Antibody (HP-F1), NovaFluor Blue 660-120S, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 737
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
Monoclonal antibody HP-F1 recognizes CD85j (ILT2, LILRB1, LIR1), a member of the ILT/LIR/MIR family. CD85j is a single transmembrane glycoprotein with four ITIMs that signal via SHP-1. It is expressed on myeloid cells (monocytes, dendritic cells) and some lymphoid subsets (NK, T, B cells). Expression correlates with leukemias (ALL, CLL) and viral infections such as HIV, EBV, and CMV. Ligands include MHC Class I molecules (HLA-G, A, F, B27, E, F). HP-F1 reduces CD16-dependent cytolytic activity of NK cells.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometry of normal human peripheral blood cells. Recommended use: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Note:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for optimal performance.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):
Required for optimal staining with NovaFluor conjugates. Add 5 µL per sample to antibody cocktails before combining with cells. For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor Conjugates:
Based on Phiton™ technology with engineered nucleic acid dye structures, designed to minimize spillover and spread.

• Excitation: 509 nm
• Emission: 665 nm
• Laser: 488 nm (Blue)

Target Information

This gene belongs to the leukocyte immunoglobulin-like receptor (LIR) family located at 19q13.4. The encoded protein (subfamily B) includes extracellular immunoglobulin domains, a transmembrane region, and ITIM motifs. It binds MHC class I molecules on antigen-presenting cells, transducing inhibitory signals to regulate immune response, inflammation, and cytotoxicity. Multiple transcript variants exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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