Thermo Fisher Scientific Osteopontin Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KRKANDESNE HSDVIDSQEL SKVSREFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVN.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Osteopontin. Anti-Human Osteopontin-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human Osteopontin by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Osteopontin (500-P314) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human Osteopontin.

Western Blot:
To detect Human Osteopontin by Western blot analysis, use at 0.1–0.2 mg/mL. When used with compatible development reagents, the detection limit for Recombinant Human Osteopontin is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P314BT-1MG is provided as 2 × 500 µg.

Target Information

Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease, and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases near the integrin-binding RGD sequence modulates OPN function and integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors, providing a molecular niche with survival advantage and serving as substrates for plasmin and cathepsin D.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.