Thermo Fisher Scientific CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.5-2.0 µg/mL

-

Product Specifications

Species Reactivity

Rat

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Rat SDF-1alpha (CXCL12)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930347

Product Specific Information

AA Sequence of recombinant protein: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat SDF-1alpha (CXCL12). Anti-Rat SDF-1alpha (CXCL12)-specific antibody was purified by affinity chromatography employing an immobilized Rat SDF-1alpha (CXCL12) matrix.

Sandwich ELISA: To detect Rat SDF-1alpha (CXCL12) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat SDF-1alpha (CXCL12) (500-P315Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat SDF-1alpha (CXCL12).

Western Blot: To detect Rat SDF-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat SDF-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.