Thermo Fisher Scientific CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• AA Sequence of recombinant protein: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat SDF-1α (CXCL12). Anti-Rat SDF-1α (CXCL12)-specific antibody was purified by affinity chromatography and biotinylated.
• Sandwich ELISA: To detect Rat SDF-1α by sandwich ELISA (100 µL/well), use 0.25–1.0 µg/mL of this antibody. When used with PeproTech Polyclonal Anti-Rat SDF-1α (500-P315) as a capture antibody, the detection limit is 0.2–0.4 ng/well of Recombinant Rat SDF-1α.
• Western Blot: For Western Blot detection of Rat SDF-1α, use 0.1–0.2 µg/mL of this antibody. Detection limit is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
• Package: 500-P315BT-1MG is provided as 2 × 500 µg.

Target Information

CXCL12 is a stromal cell-derived alpha chemokine belonging to the intercrine family. Together with its receptor CXCR4, it activates lymphocytes and is implicated in cancer metastasis, including breast cancer. Mutations in this gene are associated with resistance to HIV-1 infection. Multiple transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.