Thermo Fisher Scientific CDK2 Monoclonal Antibody (1A6)
CDK2 단백질 검출용 마우스 단일클론 항체로, WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합합니다. 인간 및 마우스 시료에 반응하며, 고순도의 Protein G 정제 항체입니다. 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | – |
| Flow Cytometry (Flow) | 1:200–1:400 | – |
| ELISA | 1:10,000 | – |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A6 |
| Immunogen | Purified recombinant fragment of human CDK2 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538524 |
Product Specific Information
MA5-17052 targets CDK2 in indirect ELISA, FACS, ICC, IHC, IF, and WB applications and shows reactivity with Human and Mouse samples.
The immunogen is a purified recombinant fragment of human CDK2 expressed in E. coli.
MA5-17052 detects CDK2, which has a predicted molecular weight of approximately 33.9 kDa.
Target Information
CDK2/cyclinO is a member of the cyclin-dependent protein kinase family complexed to CyclinO. CyclinO refers to a specific protein isoform name for the UNG gene localized in the nucleus. CyclinO exhibits significant uracil-DNA glycosylase activity.
CDK2/cyclinO protein levels increase during the G1 phase and are turned over throughout the cell cycle. The levels of cyclin A–cdk2 peak at the G1/S transition, and both cdk2 and cyclin A associate with DNA in the initiation complex during replication.
Rb protein acts as a substrate for cdk2–cyclin E and/or cdk2–cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1, S, and G2 phases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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