Thermo Fisher Scientific CD8 alpha Monoclonal Antibody (RAVB3)

Applications

Flow Cytometry (Flow)

• Tested Dilution: 1:200–1:400

ELISA

• Tested Dilution: 1:10,000

Product Specifications

Product Specific Information

MA5-17048 targets CD8 in FACS and indirect ELISA applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human CD8 expressed in E. coli.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein belonging to the immunoglobulin family of receptors. It plays a critical role in signal transduction, T cell differentiation, and activation.

CD8 is mainly expressed on T cells as a disulfide-linked heterodimer of CD8α and CD8β, functioning as a co-receptor with T cell receptor (TCR) for MHC class I molecules. Its counterpart, CD4, acts as a co-receptor for MHC class II molecules.

CD8α is essential for MHC-I binding and is also found as a homodimer on subsets of T cells, NK cells, monocytes, and dendritic cells. Upon MHC-I/peptide complex ligation, CD8 recruits Lck kinase, leading to lymphokine production, motility, and activation of cytotoxic T lymphocytes (CTLs). Activated CTLs are crucial for pathogen and tumor cell clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.