Thermo Fisher Scientific IL-35 Recombinant Rabbit Monoclonal Antibody (6H15L18)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-2 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

6H15L18

Immunogen

Recombinant protein corresponding to amino acids 57-186 of human interleukin 35 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL-35,uniProtId:P29459-1,ncbiNodeId:9606,antigenRange:57-186,antigenLength:219,antigenImageFileName:701101_IL-35_P29459-1_Rabbit.svg,antigenImageFileNamePDP:701101_IL-35_P29459-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532388

Product Specific Information

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

IL-35, or Interleukin 35 is a cytokine belonging to the IL12 family, that is produced by regulatory T cells. It is a dimeric protein composed of IL-12-alpha and IL-27-beta chains. Members of this class of cytokines are important in influencing T helper cell differentiation and supressing inflammatory response. IL-35 is known to induce proliferation of regulatory T cells, and reduce the activity of helper Th17 cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.