Thermo Fisher Scientific IkB alpha Recombinant Rabbit Monoclonal Antibody (6H7L22)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Intact IgG appears on a non-reducing gel as a ~150 kDa band and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Enhanced specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broader immunoreactivity due to large rabbit immune repertoire

Target Information

IkB-alpha is a 40 kDa protein that inhibits NF-kappaB activity through protein-protein interaction in the cytosol. This interaction masks the nuclear localization sequence of NF-kappaB, preventing its translocation to the nucleus.

Various stimuli such as TNF-alpha, IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, and other stresses can activate NF-kappaB by promoting IkB-alpha degradation.

In humans, the gene is located on chromosome 14q. Activation of NF-kappaB requires phosphorylation of IkB on specific serine residues, leading to its degradation. IkB kinase alpha (IKK alpha, also known as CHUK) interacts with and phosphorylates IkB-alpha at Ser32 and Ser36, triggering degradation.

IKK alpha plays a critical role in NF-kappaB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK alpha is stimulated by NFkB-inducing kinase (NIK), a central regulator of NF-kappaB activation in response to TNF and IL-1. The functional IKK complex includes IKK alpha, IKK beta, and IKK gamma, each contributing to IkB phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.