Thermo Fisher Scientific IKK beta Monoclonal Antibody (10AG2)

Applications

Tested Dilution

Publications

Western Blot (WB)

2-4 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500

-

Immunocytochemistry (ICC/IF)

2-5 µg/mL

-

Flow Cytometry (Flow)

0.25-0.5 µg per million cells

-

Immunoprecipitation (IP)

1:10-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

10AG2

Immunogen

full-length human IKKbeta recombinant protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IKK beta,uniProtId:O14920-1,ncbiNodeId:9606,antigenRange:1-756,antigenLength:756,antigenImageFileName:MA5-16154_IKK_beta_O14920-1_House_mouse.svg,antigenImageFileNamePDP:MA5-16154_IKK_beta_O14920-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537673

Product Specific Information

Suggested positive control: Daudi, HeLa, NIH-3T3.

Target Information

Phosphorylation of serine residues on the I-kappa-B proteins by IKK kinases, including IKBKB, marks them for destruction via the ubiquitination pathway, allowing activation of the NF-kappa-B complex. Inhibitor of nuclear factor kappa-B kinase subunit beta (IKK beta) is a serine kinase that plays an essential role in the NF-kaapa-B signaling pathway which is activated by mulitple stimuli such as inflammatory cytokines, bacterial, or viral products, DNA damages, or other cellular stresses. IKK beta acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Mutations affecting the IKK beta gene can result in Immunodeficiency 15 (IMD15).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.