Thermo Fisher Scientific IKK alpha Monoclonal Antibody (14A231)
IKK alpha 단백질을 인식하는 Mouse Monoclonal Antibody(14A231)로, Human, Mouse, Rat, Non-human primate에 반응합니다. WB, IHC, ICC, Flow, IP, ChIP 등 다양한 응용에 사용 가능하며, Protein G로 정제된 1 mg/mL 액상 항체입니다. 연구용으로만 사용하십시오.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | 1:10 |
| Flow Cytometry (Flow) | 0.25–0.5 µg per million cells |
| Immunoprecipitation (IP) | 1–2 µg/mL |
| ChIP assay (ChIP) | 1:10–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14A231 |
| Immunogen | His-tagged full-length human IKK alpha protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537676 |
Product Specific Information
- Suggested positive control: Daudi, NIH-3T3, or Raw.
Target Information
CHUK (IKK alpha) is a serine/threonine kinase involved in signal transduction and cellular communication. It is part of the cytokine-activated protein complex that inhibits the NF-kappa B complex. IKK alpha, together with IKK beta and regulatory protein NEMO (IKK gamma), phosphorylates IkB proteins, targeting them for ubiquitin-mediated degradation. It plays important roles in inflammation and cell survival.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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