Thermo Fisher Scientific IKK alpha Monoclonal Antibody (14A231)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

1:10

-

Flow Cytometry (Flow)

0.25-0.5 µg per million cells

-

Immunoprecipitation (IP)

1-2 µg/mL

-

ChIP assay (ChIP)

1:10 -1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

14A231

Immunogen

His-tagged full-length human IKK alpha protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IKK alpha,uniProtId:O15111-1,ncbiNodeId:9606,antigenRange:1-745,antigenLength:745,antigenImageFileName:MA5-16157_IKK_alpha_O15111-1_House_mouse.svg,antigenImageFileNamePDP:MA5-16157_IKK_alpha_O15111-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537676

Product Specific Information

Suggested positive control: Daudi, NIH-3T3, or Raw.

Target Information

CHUK (IKK alpha) is a serine/threonine kinase involved in signal transduction and cellular communication. It belongs to the cytokine activated protein complex that is an inhibitor of the NF-kappa B complex. IKK alpha along with IKK beta and a regulatory protein NEMO (IKK gamma) phosphorylates IkB proteins which targets the later for ubiquitin mediated degradation. It is involved with several cellular processes with extensive studies on its role in inflammation and cell survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.