Thermo Fisher Scientific EGFR (Epidermal Growth Factor Receptor) Recombinant Rabbit Monoclonal Antibody (GFR, 2968R)
EGFR 단백질을 표적으로 하는 Thermo Fisher Scientific의 재조합 토끼 모노클로날 항체입니다. Western blot, IHC, Peptide array 등 다양한 응용에 사용 가능하며, 고순도 Protein A/G 정제 방식으로 제조되었습니다. 인간 EGFR에 특이적으로 반응하며 연구용으로 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | GFR, 2968R |
| Immunogen | Recombinant full-length human EGFR protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
EGFR (Epidermal Growth Factor Receptor) is a receptor tyrosine kinase that mediates signaling in response to various growth factors. Overexpression has been associated with numerous cancers and EGFR is targeted by biological and small molecule therapeutics.
The EGFR gene is located on chromosome 7 in humans and belongs to the HER/ERbB family, which includes ERbB2, ERbB3, and ERbB4. EGFR is a transmembrane receptor; ligand binding (e.g., EGF, TGFα) induces dimerization and autophosphorylation of cytoplasmic tyrosine residues.
Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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