Thermo Fisher Scientific EGFR (Epidermal Growth Factor Receptor) Recombinant Rabbit Monoclonal Antibody (GFR, 2968R)
EGFR 단백질을 표적으로 하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체입니다. Western blot, IHC 등 다양한 응용에 적합하며, 높은 특이성과 재현성을 제공합니다. 인간 EGFR에 반응하며, 연구용으로만 사용됩니다.
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Thermo Fisher Scientific EGFR (Epidermal Growth Factor Receptor) Recombinant Rabbit Monoclonal Antibody (GFR, 2968R)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | GFR, 2968R |
| Immunogen | Recombinant full-length human EGFR protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed), incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
EGFR (Epidermal Growth Factor Receptor) is a receptor tyrosine kinase that mediates signaling in response to various growth factors. Overexpression is associated with numerous cancers and EGFR serves as a target for biological and small molecule therapeutics.
The EGFR gene is located on chromosome 7 in humans and belongs to the HER/ERbB family, which includes ERbB2, ERbB3, and ERbB4.
EGFR is a transmembrane receptor; upon ligand binding (e.g., EGF, TGF-α), it dimerizes and autophosphorylates tyrosine residues in the cytoplasmic domain.
Overexpression has been observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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