Thermo Fisher Scientific H3K4me3 Polyclonal Antibody
H3K4me3에 특이적인 Rabbit Polyclonal Antibody로, Western blot, IHC, ELISA, ChIP, ChIP-Seq 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 고순도 친화성 크로마토그래피 정제. PBS 및 글리세롤 기반 저장 버퍼, -20°C 보관.
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Thermo Fisher Scientific H3K4me3 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| ChIP assay (ChIP) | 5 µg antibody for 5–10 µg of Chromatin |
| ChIP-sequencing (ChIP-Seq) | 1:20–1:100 |
| Dot blot (DB) | 1:500–1:2,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1–100 of human histone H3 (NP_003520.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.14 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914381 |
Product Specific Information
- Positive test controls: HeLa, C2C12, C6
- Target localization: Chromosome, Nucleus
- Immunogen sequence: ARTKQTARKS TGGKAPRK
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. Together with four core histone proteins, it binds to DNA forming the nucleosome structure. Histones are essential for transcription regulation, DNA repair, replication, and chromosomal stability. The N-terminal tail of histone H3 undergoes various post-translational modifications, such as methylation, acetylation, and phosphorylation, influencing chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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