Thermo Fisher Scientific Histone H3 Polyclonal Antibody
Histone H3 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot, IHC, ELISA, IP, ChIP 등 다양한 응용에 적합하며, 고순도의 Affinity Chromatography 정제 항체입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg of whole cells |
| ChIP assay (ChIP) | 5 µg antibody for 5–10 µg of Chromatin |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 50–136 of human HIST3H3 (NP_003484.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.01 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914380 |
Product Specific Information
- Positive test controls: HeLa, HepG2, Raji, Mouse liver, Rat lung
- Subcellular localization: Chromosome, Nucleus
- Immunogen sequence:
REIRRYQKST ELLIRKLPFQ RLMREIAQDF KTDLRFQSSA VMALQEACES YLVGLFEDTN LCVIHAKRVT IMPKDIQLAR RIRGERA
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Along with other core histones, it forms nucleosomes that package and organize DNA. Histone H3 plays essential roles in transcription regulation, DNA repair, replication, and chromosomal stability. The N-terminal tail of H3 undergoes various post-translational modifications (methylation, acetylation, phosphorylation) that influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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