Thermo Fisher Scientific H4K20me2 Polyclonal Antibody
H4K20me2에 특이적인 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC, ELISA에 적합합니다. Human, Mouse, Rat 시료에 반응하며, 고순도의 Affinity Chromatography 정제 제품입니다. -20°C 보관, 연구용 전용입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic dimethylated peptide around K20 of human Histone H4 (NP_003529.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914384 |
Product Specific Information
- Positive test controls: HeLa, NIH/3T3, C6
- Subcellular localization: Chromosome, Nucleus
- Immunogen sequence: HRKVL
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules each of H2A, H2B, H3, and H4 form an octamer around which approximately 146 bp of DNA is wrapped, forming nucleosomes. The linker histone H1 interacts with linker DNA and contributes to chromatin compaction.
The gene encoding histone H4 is intronless, lacks polyA tails, and contains a palindromic termination element. It is located within the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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