Thermo Fisher Scientific Cyp4a10 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:2,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Immunohistochemistry (Frozen) (IHC (F))

1:100

-

Immunocytochemistry (ICC/IF)

1:20-1:200

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Cat, Human, Rabbit, Rat

Published species

Hamster, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues D(431) P S R F A P D S P R H S H S(445) of rat Cytochrome P450 4A. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cyp4a10,uniProtId:P08516-1,ncbiNodeId:10114,antigenRange:432-445,antigenLength:509,antigenImageFileName:PA3-033_Cyp4a10_P08516-1_Rabbit.svg,antigenImageFileNamePDP:PA3-033_Cyp4a10_P08516-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum, PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325980

Product Specific Information

PA3-033 detects cytochrome P450 4A (isoforms 1, 2, & 3) from human, feline, rabbit and rat tissues.

PA3-033 has been successfully used in Western blot, ICC/IF and IHC procedures. By Western blot, this antibody detects an ~50 kDa protein representing P450 4A in rat liver extract. Immunohistochemical staining of P450 4A in rat brain with PA3-033 results in perivascular and surrounding cortex staining.

The PA3-033 immunogen is a synthetic peptide corresponding to residues D(431) P S R F A P D S P R H S H S(445) of rat Cytochrome P450 4A.

Target Information

The cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA`s which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and w-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). 20-HETE is converted from AA by the 4A family of P450 proteins which includes at least 8 different, though closely related isoforms. 4A1, 4A2, & 4A3 have been cloned from liver, kidney and testis and have been detected in renal, hepatic & brain microvessels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.