Thermo Fisher Scientific Ly-49E/F Monoclonal Antibody (CM4), APC, eBioscience

Thermo Fisher Scientific Ly-49E/F Monoclonal Antibody (CM4), APC, eBioscience™

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.25 µg/test
  View 3 publications

Product Specifications

Additional Formats

• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This CM4 monoclonal antibody reacts with a subset of mouse Ly49 receptors, specifically Ly49E and Ly49F.
The Ly49 family of receptors are clustered in the Natural Killer Cell complex on chromosome 6. Expression is confined to NK cells, which play key roles in defense against infection, malignancy, graft rejection, and autoimmune diseases.

Ly49E is unique among family members as it contains an ITIM (immunoreceptor tyrosine-based inhibitory motif) domain and is expressed on all mouse strains. Developmentally, Ly49E is one of the first markers detected on NK cells, but its expression decreases after birth to about 1% of NK cells in the adult spleen. Upon stimulation, expression can be re-induced on Ly49E-negative cells.

The CM4 antibody is useful for characterizing Ly49E expression when used with other Ly49 antibodies such as:

• 14B11 (Ly49C/I/F/H)
• 3D10 (Ly49C)
• YLI-90 (Ly49I)
• A1 (Ly49A)

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test (100 µL final volume).
Cell number: 10⁵–10⁸ cells/test (empirically determined).
Careful titration recommended for optimal assay performance.

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The Ly49 family of receptors are clustered in the Natural Killer Cell complex on chromosome 6. Expression is confined to NK cells, which are crucial for protection from infection, malignant diseases, graft rejection, and autoimmune disorders. Ly49E contains an ITIM domain and is expressed across all mouse strains. Developmentally, Ly49E appears early on NK cells but declines postnatally; it can be re-induced upon stimulation.
The CM4 antibody enables detailed characterization of Ly49E expression when used with complementary Ly49 antibodies (14B11, 3D10, YLI-90, A1).

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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