Thermo Fisher Scientific CD369 (Clec7a, Dectin-1) Monoclonal Antibody (bg1fpj), APC, eBioscience
마우스 CD369(Clec7a, Dectin-1)을 인식하는 단클론 항체로, APC 형광체가 결합되어 있습니다. 흐름세포분석(Flow Cytometry)에 적합하며, 0.125 µg/test 권장. 면역세포 표면의 Dectin-1 발현 분석에 유용합니다. 4°C 보관, 동결 금지.
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- pk
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Thermo Fisher Scientific CD369 (Clec7a, Dectin-1) Monoclonal Antibody (bg1fpj), APC, eBioscience™
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.125 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | bg1fpj |
| Immunogen | Baculovirus-expressed Fc-containing protein |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573232 |
Available Formats
Product Specific Information
The monoclonal antibody bg1fpj recognizes mouse CD369, also known as Dectin-1 or CLEC7A, a type II transmembrane protein in the C-type lectin family.
Its ligand is β1,3-glucan, a key polysaccharide component found in fungal, plant, and some bacterial cell walls. CD369 binds to organisms such as Candida, Aspergillus, Pneumocystis, Coccidioides, and Mycobacterium.
Ligand binding is calcium-dependent and signals through Syk kinase via the ITAM domain, leading to endocytosis/phagocytosis and cytokine production (IL-2, IL-6, IL-10, TNFα, IL-1β, IL-23). CD369 signaling has roles in Th17 and Th1 differentiation.
CD369 is primarily expressed on myeloid cells (neutrophils, monocytes, macrophages, dendritic cells), with lower levels on lymphocytes and mucosal epithelium.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse lysed whole blood cells at ≤0.125 µg/test.
A test is defined as the amount of antibody staining a 100 µL cell sample (10⁵–10⁸ cells/test). Titrate for optimal performance.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CLEC7A (Dectin-1) belongs to the C-type lectin-like domain (CTL/CTLD) superfamily and is mainly expressed on myeloid cells.
It is a type II membrane glycoprotein receptor with an extracellular C-type lectin-like domain and a cytoplasmic ITAM motif.
CLEC7A acts as a pattern-recognition receptor for β-1,3- and β-1,6-linked glucans from fungi and plants, playing a crucial role in innate immune responses. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, inducing reactive oxygen species formation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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